Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool† †Dedicated to the memory of Joachim H. G. Steinke. ‡ ‡Electronic supplementary information (ESI) available: Further experimental data. See DOI: 10.1039/c3sm00131hClick here for additional data file.

Self-assembling α,γ-cyclic peptides that generate cavities with tunable properties† †Electronic supplementary information (ESI) available: Synthetic experimental details, DFT calculation details, additional figures (relevant NMR data) and schemes (synthetic schemes for peptide and amino acid preparation). CCDC 1400134. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc03187g Click here for additional data file. Click here for additional data file.

Stretchable supramolecular hydrogels with triple shape memory effect† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6sc02354a Click here for additional data file. Click here for additional data file. Click here for additional data file. Click here for additional data file.

Incorporation of native antibodies and Fc-fusion proteins on DNA nanostructures via a modular conjugation strategy† †Electronic supplementary information (ESI) available: Experimental methods, DNA origami design, DNA sequences, and additional experimental data. See DOI: 10.1039/c7cc04178k

A photocrosslinkable protein G variant was used as an adapter protein to covalently and site-specifically conjugate an antibody and an Fc-fusion protein to an oligonucleotide. This modular approach enables straightforward decoration of DNA nanostructures with complex native proteins while retaining their innate binding affinity, allowing precise control over the nanoscale spatial organization o...

Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc03150e Click here for additional data file.

Genetically encoded optical activation of DNA recombination in human cells† †Electronic supplementary information (ESI) available: Experimental protocols. See DOI: 10.1039/c6cc03934k Click here for additional data file.

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.